FK506-binding protein 12-rapamycin complex-associated protein 1, Mammalian target of rapamycin, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, mTOR
Immunogen
KLH conjugated Synthetic peptide
corresponding to Human mTOR
Isotype
IgG
Purity
Affinity purification
Subcellular location
Cytoplasm, Nucleus
Predicted MW.
289 kDa
Observed MW.
289 kDa
Uniprot ID
P42345, Q9JLN9, P42346
Applications
Applications
Species
Dilution
Positive Tissue
WB
Human, Mouse, Rat
1: 400-1: 800
heart, liver, brain
Background
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications.
Images
Western blot analysis of mTOR (GB111840)
at dilution of 1: 800
Lane 1: HEK293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Mouse heart tissue lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat heart tissue lysate
Lane 8: Rat liver tissue lysate
Lane 9: Rat brain tissue lysate
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).